Western blot analysis of eIF4E in extracts of various cell lines. Samples were incubated with eIF4E Monoclonal antibody (Product # MA5-35225) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Western Blot analysis of eIF4E performed on various cell lines using a eIF4E monoclonal antibody (Product # MA5-35225) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.
Supplier Page from Thermo Fisher Scientific for eIF4E Antibody